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pucx multiple cloning site  (Twist Bioscience)


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    Structured Review

    Twist Bioscience pucx multiple cloning site
    Pucx Multiple Cloning Site, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pucx+multiple+cloning+site/pucx+multiple+cloning+site/pmc10055417-184-30-19
    Average 90 stars, based on 1 article reviews
    pucx multiple cloning site - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Cloning:

    Article Title: A metagenomic library cloning strategy that promotes high-level expression of captured genes to enable efficient functional screening
    Article Snippet: For replacement of the antibiotic resistance gene, the pUCX vector was amplified with forward primer CTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAAC and reverse primer ACTCTTCCTTTTTCAATATTATTGAAGC and assembled with a synthetic gentamycin cassette ordered from Twist Bioscience containing 5′ and 3′ 20-bp pUCX homology arms, using NEBuilder ® HiFi DNA Assembly (New England Biolabs). .. A synthetic cloning site comprising an NcoI recognition sequence flanked by XbaI and HindIII restriction sites was ordered from Twist Bioscience and used to replace the XbaI-HindIII region of the pUCX multiple cloning site by restriction cloning. ..

    Sequencing:

    Article Title: A metagenomic library cloning strategy that promotes high-level expression of captured genes to enable efficient functional screening
    Article Snippet: For replacement of the antibiotic resistance gene, the pUCX vector was amplified with forward primer CTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAAC and reverse primer ACTCTTCCTTTTTCAATATTATTGAAGC and assembled with a synthetic gentamycin cassette ordered from Twist Bioscience containing 5′ and 3′ 20-bp pUCX homology arms, using NEBuilder ® HiFi DNA Assembly (New England Biolabs). .. A synthetic cloning site comprising an NcoI recognition sequence flanked by XbaI and HindIII restriction sites was ordered from Twist Bioscience and used to replace the XbaI-HindIII region of the pUCX multiple cloning site by restriction cloning. ..



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    90
    Twist Bioscience pucx multiple cloning site
    Pucx Multiple Cloning Site, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pucx+multiple+cloning+site/pucx+multiple+cloning+site/pmc10055417-184-30-19
    Average 90 stars, based on 1 article reviews
    pucx multiple cloning site - by Bioz Stars, 2026-09
    90/100 stars
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